Accueil Sciences et Innovations Sciences Towards a commercial production of phage-based bioactive paper

Towards a commercial production of phage-based bioactive paper

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Article technique paru dans J-FOR* Vol.1, No1, 2011 (Disponible en anglais seulement)

RÉSUMÉ

Detection and disabling of harmful bacteria such as Escherichia coli (E.coli), Listeria, and Salmonella is of great importance to control disease apparition in air, water or food. Due to inherent flexibility, low cost and ease of fabrication, papers and non-woven are ideal substrates to functionalize for bioactivity.

In this work, we show that an E.coli bacteria biosensor can be manufactured by immobilizing bacteriophages onto paper surface through conventional high-speed printing and coating techniques. We found the bioactivity of papers impregnated with a phage solution decreases rapidly in time when stored in ambient humidity conditions. The decrease can be alleviated through the use of gelatine in the coating colour applied to the paper, however. We also demonstrate that sufficient levels of paper bioactivity can be recovered through paper rewetting: initial bioactivity can even be recovered after four rewetting/drying cycles. We also demonstrate how conventional gravure printing can be used to transfer phage onto the paper surface due to the role of paper porosity and permeability for phage retention at the paper surface. Printing press pressure and speed affect phage transfer and their subsequent biological efficiency. Finally, when coating a mixture of phages and gelatine on the paper surface at high speed, we demonstrate that phages withstand the high intensity IR drying associated with the coating process. Gelatine application noticeably increases the lifetime of bioactivity, thus rendering feasible large-scale commercial production of bioactive paper.

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AUTEURS :

ART1-auteur1 ART1-auteur2 ART1-auteur3 ART1-auteur4
 TARIK JABRANE
Centre de Recherche
sur les Matériaux
Lignocellulosiques,
Université du Québec
à Trois-Rivières
Trois-Rivières, Qc,
Canada
 MARTIN DUBÉ
Centre de Recherche
sur les Matériaux
Lignocellulosiques,
Université du Québec
à Trois-Rivières
Trois-Rivières, Qc,
Canada
MANSELL GRIFFITHS
Department of Food
Science,
University of Guelph
Guelph, On,
Canada 
PATRICE J. MANGIN
Centre de Recherche
sur les Matériaux
Lignocellulosiques,
Université du Québec
à Trois-Rivières
Trois-Rivières, Qc,
Canada 

* J-FOR - The Journal of Science & Technology for Forest Products and Processes, est une publication phare de PAPTAC, comprenant des articles évalués de haute qualité, traitant de technologie et de science relativement à des domaines traditionnels et émergents propres à l'industrie forestière.

J-FOR-1

 
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